Secretome

Co-investigator: Prof. Galderisi Umberto, Dr Alessio Nicola, Dr. Aprile Domenico, Dr Demirsoy Ibrahim 

Senescence-associated secreted proteins (SASP)

In the last years, I have focused on the secretome of senescent cells. It is evident that senescence process may greatly affect also the composition of mesenchymal stem cells (MSC) secretome through a shift from functional paracrine signalling to production of senescent-associated secreted factors that have potent autocrine and paracrine activities. Changes in secretome profiles of MSC may great impair their activities, which depends on the capability to secrete many factors, like cytokines and chemokines.

In this setting, we have decided to carry out a comparative analysis of MSC secretome from young and senescent cultures and evaluate if factors secreted from old MSC cultures may induce senescence or arrest proliferation, or promote cytotoxic effects in young cells.

OUR Publications:

1: Severino V, Alessio N, Farina A, Sandomenico A, Cipollaro M, Peluso G, Galderisi U, Chambery A. Insulin-like growth factor binding proteins 4 and 7 released by senescent cells promote premature senescence in mesenchymal stem cells. Cell Death Dis. 2013 Nov 7;4:e911. doi: 10.1038/cddis.2013.445. PubMed PMID: 24201810; PubMed Central PMCID: PMC3847322.

2: Özcan S, Alessio N, Acar MB, Toprak G, Gönen ZB, Peluso G, Galderisi U. Myeloma cells can corrupt senescent mesenchymal stromal cells and impair their anti-tumor activity. Oncotarget. 2015 Nov 24;6(37):39482-92. doi: 10.18632/oncotarget.5430. PubMed PMID: 26498687; PubMed Central PMCID: PMC4741840.

3: Özcan S, Alessio N, Acar MB, Mert E, Omerli F, Peluso G, Galderisi U. Unbiased analysis of senescence associated secretory phenotype (SASP) to identify common components following different genotoxic stresses. Aging (Albany NY). 2016 Jul;8(7):1316-29. doi: 10.18632/aging.100971. PubMed PMID: 27288264; PubMed Central PMCID: PMC4993333.

4: Alessio N, Özcan S, Tatsumi K, Murat A, Peluso G, Dezawa M, Galderisi U. The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation. Cell Cycle. 2017 Jan 2;16(1):33-44. doi: 10.1080/15384101.2016.1211215. Epub 2016 Jul 27. PubMed PMID: 27463232; PubMed Central PMCID: PMC5270533.