name: Domenico surname: Aprile
Educational and Training
- 2018 Master degree in Biological Sciences, summa cum laude, University of Naples Federico II, Naples, Italy.
- PhD student in Clinical and Experimental Medical Sciences XXXIV cycle, University of "Campania Luigi Vanvitelli"
Main Work Experiences:
- 2017 Internship and master's thesis during Erasmus Plus Program at Institute of Molecular Medicine (IMM), University of Lisbon, Lisbon, Portugal.
Erasmus Plus at Pablo de Olavide University,
Protein extraction and purification, SDS-PAGE, Immunoprecipitation, Western blotting.
DNA extraction and purification, RNA extraction and purification, PCR, RT-PCR, Real time PCR, DNA digestion with restriction enzymes,
Isolation and cultivation of different stem cell line from several organs both human and mouse.
In vitro cultures of mesenchymal stem cells and of stabilized tumour cell lines, biological assays to detect cellular proliferation, apoptosis and senescence.
Immunocytochemistry and Immonohistochemestry
Computer skills: WIN, MAC, Word, Excel, Explorer,
Outlook, Nero Burning, Cd Creator, RealOne Media Player, PowerPoint,
Photoshop; Molecular Analyst BioRad, Quantity One BioRad, Primer Express,
Main current research interests:
- Basic and applied researches on normal and cancer stem cells.
- Analysis of senescence processes that affect stem cell properties.
- Effect of low dose radiations on the biology of normal and cancer stem cells.
- Effects of sera from obesity and natural skinny people on MSCs biology
1: Pólvora-Brandão D, Joaquim M, Godinho I, Aprile D, Álvaro AR, Onofre I, Raposo AC, Pereira de Almeida L, Duarte ST, da Rocha ST. Loss of hierarchical imprinting regulation at the Prader-Willi/Angelman syndrome locus in human iPSCs. Hum Mol Genet. 2018 Dec 1;27(23):3999-4011. doi: 10.1093/hmg/ddy274. PubMed PMID: 30102380; PubMed Central PMCID: PMC6240739.
2: Nicola Alessio, Domenico Aprile, Tiziana Squillaro, Giovanni Di Bernardo, Mauro Finicelli, Mariarosa AB Melone, Gianfranco Peluso, Umberto Galderisi. Senescence associated secretory phenotype (SASP) from mesenchymal stromal cells impairs growth of transformedprostate cells but has no effect on metastatic prostatic cancer cells. Submitted to Cancers.
3: Nicola Alessio, Elisa Santoro, Tiziana Squillaro, Domenico Aprile, Massimo Briccola, Paolo Giubbini, Raffaella Marchesani, Maria Rosaria Muoio, Monica Lamberti. Low-level radiofrequency exposure does not induce changes in MSC biology: an in vitro study for the prevention of NIR-related damage. Submitted to Stem Cells and Cloning: Advances and Applications.