name: Tiziana surname: Squillaro

Telephone: 0815667508

e.mail: tiziana.squillaro@unicampania.it

Nationality: ITALY

Educational and Training 

Studies: 

  • 2005 Master degree in Biological Sciences, University of Naples "Federico II", Naples, Italy.
  • 2009 PhD in Medical Genetics, Department of Molecular Biology, University of Siena, Siena, Italy

Present Position: 

  •  Postdoctoral fellow at the Department of Medical, Surgical, Neurological, Metabolic Sciences, and Aging, University of Campania "Luigi Vanvitelli", Naples, Italy.

Main Work Experiences: 

  • 2009/2010 Postdoctoral fellow at the Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.
  • 2011 Postdoctoral fellow at the Human Health Foundation, Terni, italy.
    • 2013 Postdoctoral fellow at the Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.
    • 2014/2016: Postdoctoral fellow at the Institute of Bioscience and Bioresources, CNR, Naples, Italy.
    • 2017/present Postdoctoral fellow at the Department of Medical, Surgical, Neurological, Metabolic Sciences, and Aging, University of Campania "Luigi Vanvitelli", Naples, Italy.

Skills:

Protein extraction and purification, SDS-PAGE, Immunoprecipitation, Western blotting, dot blotting. 

Recombinant DNA technology, plasmidic DNA extraction and purification, RNA extraction and purification, PCR, RT-PCR, Real time PCR, DNA digestion with restriction enzymes, 
Isolation and cultivation of different stem cell line from several organs both human and mouse.  
In vitro cultures of mesenchymal stem cells and of stabilized tumour cell lines, biological assays to detect cellular proliferation, apoptosis and senescence. 
Immunocytochemistry and Immonohistochemestry
Transfection cells with different vectors like the virus and lipofectamine. 

Computer skills: WIN, MAC, Word, Excel, Explorer, Outlook, Nero Burning, Cd Creator, RealOne Media Player, PowerPoint, Photoshop; Molecular Analyst BioRad, Quantity One BioRad, Primer Express, Oligo 4.0.

Research interests:

Main current research interests:

  • Basic and applied researches on normal and cancer stem cells.
  • Analysis of senescence processes that affect stem cell properties.
  • Effect of low dose radiations on the biology of normal and cancer stem cells.
  • Secretome effects of Mesenchymal stromal cells
  • RETT syndrome 

Selected Publications:


She is co-author of 27 articles (H-index 11, citations 569 by Scopus)

1: Alessio N, Stellavato A, Squillaro T, Del Gaudio S, Di Bernardo G, Peluso G, De Rosa M, Schiraldi C, Galderisi U. Hybrid complexes of high and low molecular weight hyaluronan delay in vitro replicative senescence of mesenchymal stromal cells: a pilot study for future therapeutic application. Aging (Albany NY). 2018 Jul 12;10(7):1575-1585. doi: 10.18632/aging.101493. Pubmed PMID: 30001217.

2: Squillaro T, Galano G, De Rosa R, Peluso G, Galderisi U. Concise Review: The Effect of Low-Dose Ionizing Radiation on Stem Cell Biology: A Contribution to Radiation Risk. Stem Cells. 2018 Aug;36(8):1146-1153. doi: 10.1002/stem.2836. PubMed PMID: 29664142.

3: Alessio N, Squillaro T, Özcan S, Di Bernardo G, Venditti M, Melone M, Peluso G, Galderisi U. Stress and stem cells: adult Muse cells tolerate extensive genotoxic stimuli better than mesenchymal stromal cells. Oncotarget. 2018 Apr 10;9(27):19328-19341. doi: 10.18632/oncotarget.25039. eCollection 2018 Apr 10. Pubmed PMID: 29721206.

4: Squillaro T, Peluso G, Galderisi U. Clinical Trials With Mesenchymal Stem Cells: An Update. Cell Transplant. 2016;25(5):829-48. doi: 10.3727/096368915X689622. Epub 2015 Sep 29. Review. Pubmed PMID: 26423725.

5: Capasso S, Alessio N, Squillaro T, Di Bernardo G, Melone MA, Cipollaro M, Peluso G, Galderisi U. Changes in autophagy, proteasome activity and metabolism to determine a specific signature for acute and chronic senescent mesenchymal stromal cells. Oncotarget. 2015 Nov 24;6(37):39457-68. doi:10.18632/oncotarget.6277. PubMed PMID: 26540573.

6: Squillaro T, Severino V, Alessio N, Farina A, Di Bernardo G, Cipollaro M, Peluso G, Chambery A, Galderisi U. De-regulated expression of the BRG1 chromatin remodeling factor in bone marrow mesenchymal stromal cells induces senescence associated with the silencing of NANOG and changes in the levels of chromatin proteins. Cell Cycle. 2015;14(8):1315-26. doi: 10.4161/15384101.2014.995053. Pubmed PMID: 25724006.

7: Finicelli M, Benedetti G, Squillaro T, Pistilli B, Marcellusi A, Mariani P, Santinelli A, Latini L, Galderisi U, Giordano A. Expression of stemness genes in primary breast cancer tissues: the role of SOX2 as a prognostic marker for detection of early recurrence. Oncotarget. 2014 Oct 30;5(20):9678-88. Pubmed PMID: 25127259.

8: Alessio N, Squillaro T, Cipollaro M, Bagella L, Giordano A, Galderisi U. The BRG1 ATPase of chromatin remodeling complexes is involved in modulation of mesenchymal stem cell senescence through RB-P53 pathways. Oncogene. 2010 Oct 7;29(40):5452-63. doi: 10.1038/onc.2010.285. Epub 2010 Aug 9. Pubmed PMID: 20697355.

9: Alessio N, Squillaro T, Cipollaro M, Bagella L, Giordano A, Galderisi U. The BRG1 ATPase of chromatin remodeling complexes is involved in modulation of mesenchymal stem cell senescence through RB-P53 pathways. Oncogene. 2010 Oct 7;29(40):5452-63. doi: 10.1038/onc.2010.285. Epub 2010 Aug 9. Pubmed PMID: 20697355.

10: Squillaro T, Alessio N, Cipollaro M, Renieri A, Giordano A, Galderisi U. Partial silencing of methyl cytosine protein binding 2 (MECP2) in mesenchymal stem cells induces senescence with an increase in damaged DNA. FASEB J. 2010 May;24(5):1593-603. doi: 10.1096/fj.09-143057. Epub 2010 Jan 11. Erratum in: FASEB J. 2016 May;30(5):2064. Pubmed PMID: 20065105