name: Afshin surname: Samiminemati

Telephone: 0815667508

e.mail: afshin.samiminemati@unicampania.it

Nationality: Iran

Educational and Training

Studies:

2019 Master degree in Molecular Biotechnology, University of Malrk Ashtar, Teheran, Iran.
2017 Bachelor Degree in Cell and Molecualr Biology, University of Mazandaran, Babolsar, Iran.

Present Position:

PhD Student in Clinical and Experimental Medical Sciences, University of Campania "Luigi Vanvitelli", Naples, Italy.

Main Work Experiences:

2020/2022 Quality Control Expert, Arvand Pharmed, Teheran, Iran.

Skills:

Protein extraction and purification, SDS-PAGE, immunoprecipitation, Western blotting, dot blotting.

Recombinant DNA technology, plasmidic DNA extraction and purification, RNA extraction and purification, PCR, RT-PCR, Real time PCR, DNA digestion with restriction enzymes. Isolation and cultivation of different stem cell line from several organs both human and mouse. In vitro cultures of mesenchymal stem cells and of stabilized tumour cell lines, biological assays to detect cellular proliferation, apoptosis and senescence. Immunocytochemistry and Immunohistochemestry. Transfection cells with different vectors like the virus and lipofectamine. Vaccine design and formulation.

Computer skills: WIN, MAC, Microsoft Office / Google Drive / Outlook / Structural bioinformatics

Research interests:

Main current research interests:

Basic and applied researches on normal and cancer stem cells.
Analysis of senescence processes that affect stem cell properties.
Effect of low dose radiations on the biology of normal and cancer stem cells.

Selected Publications:

1. AFSHIN SAMIMINEMATI, SAKO MIRZAIE, MOHAMMAD REZA MASOUMIAN, FATEMEH SHEIKHI, MOSTAFA JAMALAN (2022) "Development a novel multi-epitope DNA vaccine against human SARS coronavirus-2: an immunoinformatics designing study," Turkish Journal of Biology: Vol. 46: No. 4, Article 1. https://doi.org/10.55730/1300-0152.2615

2. Jabarzadeh, S., Samiminemati, A. & Zeinoddini, M. In Silico Design of a New Multi-Epitope Peptide-Based Vaccine Candidate Against Q Fever. Mol Biol 55, 950–960 (2021). https://doi.org/10.1134/S0026893321050150

3. Faridfar, G., Zeinoddini, M., Akbarzedehkolahi, S., Shahin Faridfar & Afshin Samimi Nemati . Immunodiagnostic of Vibrio cholerae O1 using localized surface plasmon resonance (LSPR) biosensor. Int Microbiol 24, 115–122 (2021). https://doi.org/10.1007/s10123-020-00148-8

4. Afshin Samimi Nemati, Majid Tafrihi, Fatemeh Sheikhi, Abolfazl Rostamian Tabari. Designing a New Multiepitope-based Vaccine Against COVID-19. Res Mol Med (RMM) 2021; 9 (2) :103-118.

5. Fatemeh Yaghubi, Mehdi Zeinoddini, Ali Reza Saeedinia, Azadeh Azizi & Afshin Samimi Nemati. Design of Localized Surface Plasmon Resonance (LSPR) Biosensor for Immunodiagnostic of E. coli O157:H7 Using Gold Nanoparticles Conjugated to the Chicken Antibody. Plasmonics 15, 1481–1487 (2020). https://doi.org/10.1007/s11468-020-01162-2