name: Nicola surname: Alessio

Telephone: 0815667663

e.mail: nicola.alessio@yahoo.it

Nationality: ITALY

Educational and Training

Studies:

2007 Master degree in Biological Sciences, summa cum laude, University of Naples, Naples, Italy.
2012 PhD, Department of Biomedical Science, Biochemistry and Molecular Biology, University of Sassari, Sassari, Italy.

Present Position:

Postdoctoral fellowship in Molecular Biology at the School of Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.

Main Work Experiences:

2007/2008 Young Researcher at Human Health Foundation Onlus. Philadelphia, PA, USA.
2012/2013 Researcher at CNR (National Research Council), Napoli, Italy.
2013/2017 Postdoctoral fellowship at University of Campania, Napoli, Italy.
2013 Visiting Researcher at CNIO, Madrid, Spain .
2014 Visiting Researcher at GENEKOK, Kayseri, Turkey.
2015 Visiting Researcher at Tohoku University, Sendai, Japan.
2017 Visiting Researcher at CEA, Fontenay-aux-Roses, France.

Membership:

Member of Stem Cell Research Italy (SCR Italy)

Skills:

Protein extraction and purification, SDS-PAGE, Immunoprecipitation, Western blotting, dot blotting.

Recombinant DNA technology, plasmidic DNA extraction and purification, RNA extraction and purification, PCR, RT-PCR, Real time PCR, DNA digestion with restriction enzymes, Isolation and cultivation of different stem cell line from several organs both human and mouse.

In vitro cultures of mesenchymal stem cells and of stabilized tumour cell lines, biological assays to detect cellular proliferation, apoptosis and senescence.

Immunocytochemistry and Immonohistochemestry

Transfection cells with different vectors like the virus and lipofectamine.

Computer skills: WIN, MAC, Word, Excel, Explorer, Outlook, Nero Burning, Cd Creator, RealOne Media Player, PowerPoint, Photoshop; Molecular Analyst BioRad, Quantity One BioRad, Primer Express, Oligo 4.0.

Research interests:

Main current research interests:

Basic and applied researches on normal and cancer stem cells.
Analysis of senescence processes that affect stem cell properties.
Effect of low dose radiations on the biology of normal and cancer stem cells.
Secretome effects of Mesenchymal stromal cells
The role of the Rb family proteins in the senescence
MUSE cells

Selected Publications:

He is co-author of more than 55 articles (H-index 26, citations 1849 by Scopus )

Full pubblication list: ORCID

1: Alessio N, Stellavato A, Squillaro T, Del Gaudio S, Di Bernardo G, Peluso G, De Rosa M, Schiraldi C, Galderisi U. Hybrid complexes of high and low molecular weight hyaluronan delay in vitro replicative senescence of mesenchymal stromal cells: a pilot study for future therapeutic application. Aging (Albany NY). 2018 Jul 12;10(7):1575-1585. doi: 10.18632/aging.101493. PubMed PMID: 30001217; PubMed Central PMCID: PMC6075440.

2: Alessio N, Pipino C, Mandatori D, Di Tomo P, Ferone A, Marchiso M, Melone MAB, Peluso G, Pandolfi A, Galderisi U. Mesenchymal stromal cells from amniotic fluid are less prone to senescence compared to those obtained from bone marrow: An in vitro study. J Cell Physiol. 2018 Nov;233(11):8996-9006. doi: 10.1002/jcp.26845. Epub 2018 Jun 15. PubMed PMID: 29904927.

3: Alessio N, Squillaro T, Özcan S, Di Bernardo G, Venditti M, Melone M, Peluso G, Galderisi U. Stress and stem cells: adult Muse cells tolerate extensive genotoxic stimuli better than mesenchymal stromal cells. Oncotarget. 2018 Apr 10;9(27):19328-19341. doi: 10.18632/oncotarget.25039. eCollection 2018 Apr 10. PubMed PMID: 29721206; PubMed Central PMCID: PMC5922400.

4: Alessio N, Riccitiello F, Squillaro T, Capasso S, Del Gaudio S, Di Bernardo G, Cipollaro M, Melone MAB, Peluso G, Galderisi U. Neural stem cells from a mouse model of Rett syndrome are prone to senescence, show reduced capacity to cope with genotoxic stress, and are impaired in the differentiation process. Exp Mol Med. 2018 Mar 22;50(3):1. doi: 10.1038/s12276-017-0005-x. PubMed PMID: 29563495.

5: Alessio N, Capasso S, Ferone A, Di Bernardo G, Cipollaro M, Casale F, Peluso G, Giordano A, Galderisi U. Misidentified Human Gene Functions with Mouse Models: The Case of the Retinoblastoma Gene Family in Senescence. Neoplasia. 2017 Oct;19(10):781-790. doi: 10.1016/j.neo.2017.06.005. Epub 2017 Aug 30. PubMed PMID: 28865301; PubMed Central PMCID: PMC5577395.

6: Alessio N, Esposito G, Galano G, De Rosa R, Anello P, Peluso G, Tabocchini MA, Galderisi U. Irradiation of Mesenchymal Stromal Cells with Low and High Doses of Alpha Particles Induces Senescence and/or Apoptosis. J Cell Biochem. 2017 Mar 2. doi: 10.1002/jcb.25961. [Epub ahead of print] PubMed PMID: 28252222.

7: Alessio N, Özcan S, Tatsumi K, Murat A, Peluso G, Dezawa M, Galderisi U. The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation. Cell Cycle. 2017 Jan 2;16(1):33-44. doi: 10.1080/15384101.2016.1211215. PubMed PMID: 27463232; PubMed Central PMCID: PMC5270533.

8: Özcan S, Alessio N, Acar MB, Mert E, Omerli F, Peluso G, Galderisi U. Unbiased analysis of senescence associated secretory phenotype (SASP) to identify common components following different genotoxic stresses. Aging (Albany NY). 2016 Jul;8(7):1316-29. doi: 10.18632/aging.100971. PubMed PMID: 27288264; PubMed Central PMCID: PMC4993333.

9: Alessio N, Capasso S, Di Bernardo G, Cappabianca S, Casale F, Calarco A, Cipollaro M, Peluso G, Galderisi U. Mesenchymal stromal cells having inactivated RB1 survive following low irradiation and accumulate damaged DNA: Hints for side effects following radiotherapy. Cell Cycle. 2017 Feb;16(3):251-258. doi: 10.1080/15384101.2016.1175798. PubMed PMID: 27124644; PubMed Central PMCID: PMC5323029.19: Severino V, Alessio N, Farina A, Sandomenico A, Cipollaro M, Peluso G, Galderisi U, Chambery A. Insulin-like growth factor binding proteins 4 and 7 released by senescent cells promote premature senescence in mesenchymal stem cells. Cell Death Dis. 2013 Nov 7;4:e911. doi: 10.1038/cddis.2013.445. PubMed PMID: 24201810; PubMed Central PMCID: PMC3847322.

10: Alessio N, Bohn W, Rauchberger V, Rizzolio F, Cipollaro M, Rosemann M, Irmler M, Beckers J, Giordano A, Galderisi U. Silencing of RB1 but not of RB2/P130 induces cellular senescence and impairs the differentiation potential of human mesenchymal stem cells. Cell Mol Life Sci. 2013 May;70(9):1637-51. doi: 10.1007/s00018-012-1224-x. PubMed PMID: 23370776.